Completion of the vesico-urethral anastomosis is a critical step of robotic radical prostatectomy. This video highlights a novel technique of three-layer knotless anastomosis using a barbed and looped suture. This was a prospective single surgeon study of 21 consecutive patients who underwent three-layer anastomotic technique using a polygliconate barbed suture. The first layer of the anastomosis approximates Denonvilliers fascia to the posterior rhabdosphincter; the second layer approximates the posterior bladder neck to the posterior rhabdosphincter; the third layer is precise urethral mucosa to bladder mucosa adaptation. We evaluated anastomosis time, the rate of urinary leaks and catheterization time. Furthermore, anastomotic strictures and urinary retention rates were examined at six months follow up. Mean anastomosis time was 13 minutes (range 10 to 21 minutes). There was no anastomotic extravasation. Median catheterization time was 6 days. At 6 months, we did not observe any anastomotic stricture and/or urinary retention. The knotless three-layer technique provides a stable and tension-free anastomosis. The barbed and looped suture maintains running suture line tension and optimizes mucosa-to-mucosa reapproximation and completion of a water-tight closure. Larger experience and further follow-up will determine any benefits of this technique on anastomotic urinary leak and stricture rates.